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. 2005 Feb 14;168(4):587–598. doi: 10.1083/jcb.200410114

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Copyright © 2005, The Rockefeller University Press

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Figure 2. — Shot is recruited to microtubule tips by DmEB1. (A) Colocalization of DmEB1 (green) and Shot (red) on microtubule tips in Drosophila S2 cells. (B) Enlarged view of the yellow boxed region outlined in A detailing microtubule tip localization. Individual channels and the resulting merged image are indicated. The distribution of DmEB1 (green) and Shot (red) on individual microtubule tips designated by numbers in B are represented in C normalized against individual maximum intensity along the microtubule axis with a minus and plus denoting the polarity of the microtubule tip. (D) Control S2 cells expressing GFP-tubulin (green) and immunostained for Shot (red); colocalization of Shot at microtubule tips can be seen. (E) After DmEB1 RNAi treatment, Shot (red) is diffusely localized and no longer stains at microtubules tips or along the length of the microtubule, as shown in this representative S2 cell expressing GFP-tubulin (green). 900 cells were analyzed in total. Bars: (A) 5 μm; (C) 1 μm; (D and E) 10 μm.