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. 2005 Feb 14;168(4):587–598. doi: 10.1083/jcb.200410114

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Copyright © 2005, The Rockefeller University Press

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Figure 5. — Mutations within the coiled coil–four-helix bundle junction abrogate binding to MACF2_1595-1637. (A) Surface plasmon resonance of EB1 (FL) at various concentrations (solid lines) on a sensor chip with immobilized MACF2_1595-1637. The resulting bivalent model is overlaid in dashed lines with colors corresponding to experimental EB1 concentrations. (B) Surface plasmon resonance of wild-type and mutant full-length EB1 constructs at 2.5 μM on a sensor chip with immobilized MACF2_1595-1637. Color coding at right designates the relative effect on the association of the EB1 mutants with surface-bound peptide. Yellow, altered binding; red, no binding. (C) Gel filtration analysis of EB1 full-length wild-type protein and the EB1 I224A mutant.