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. 2005 Feb 14;168(4):607–618. doi: 10.1083/jcb.200406126

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Copyright © 2005, The Rockefeller University Press

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Figure 3. — BoNT/C-induced apoptosis is independent of the block of neurotransmitter release and is not prevented by glutamatergic stimulation. (A) Dose–response of BoNT/C-induced block of neurotransmitter release and correlation with apoptosis. Neurotransmitter release was triggered by potassium depolarization in l-[G-³H]glutamine–loaded cultures. (B) Comparison of the block of neurotransmitter release with apoptosis induced by 20 ng/ml BoNT/C and 2 μg/ml BoNT/A. (C) CGCs were incubated with 20 ng/ml BoNT/C. After 12 h, 1 μM AMPA or 0.5 μM glutamate was added, and, after 36 h, apoptosis was evaluated by H-33342 staining. (D) CGCs were incubated with 20 ng/ml BoNT/C for 15 h. Then, they were exposed to 300 μM glutamate for 1 h, in the presence or in the absence of 2 μM MK801. Apoptosis was evaluated as a percentage of condensed nuclei. Data are means ± SD for triplicate determinations.