Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Feb 14;168(4):607–618. doi: 10.1083/jcb.200406126

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — Cytoskeletal disassembly and abnormal tau phosphorylation accompanied the early degeneration of the neurites. (A) CGCs were exposed to 20 ng/ml BoNT/C for the times indicated and immunostained for actin and β_III-tubulin. Confocal images were acquired from the same field. The bulblike structures present at 8 h along the neurites are shown at higher magnification in B (arrows). (C) The total amount of tau protein was detected by the K9JA antibody, which recognizes tau independently of its phosphorylation. In the same cell extracts, abnormal tau phosphorylation was detected by using the S202/205 phosphospecific antibody 11b. Neurons were exposed to 20 ng/ml BoNT/C for the indicated times. (D) The pattern of tau distribution was determined by a different antibody (Innogenetics) and changed in cells exposed to 20 ng/ml BoNT/C. Images in A, B, and D are extended focus images derived by merging 50–60 focal planes acquired in the z dimension with a 63× oil 1.3 NA apochromat objective. Bars, 20 μm.