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. 2005 Mar 14;168(6):855–862. doi: 10.1083/jcb.200411026

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Copyright © 2005, The Rockefeller University Press

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Figure 1. — Pathogenic and substrate trap forms of torsinA display reduced mobility in the NE. (A) GFP immunolabeling of BHK_GFPWT, BHK_GFPΔE, and BHK_GFPEQ stable cell lines. (B and D) GFP fluorescence of BHK21 cells transiently transfected with GFPWT-, GFPΔE-, or GFPEQ-torsinA and DsRed fluorescence of control cells transfected with DsRed2-ER (CLONTECH Laboratories, Inc.). Images show representative cells immediately before (top), immediately after (middle), and 120 s after (bottom) bleaching a ROI (boxed areas) in the NE (B) or ER (D). Bars, 10 μm. (C and E) Relative fluorescence intensity in the ROI as a function of time after photobleaching at time point “B” (B, bleach; see Materials and methods). Points show mean values and SEM.