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. 2005 Mar 14;168(6):929–939. doi: 10.1083/jcb.200407148

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Copyright © 2005, The Rockefeller University Press

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Figure 2. — Destaining of weakly labeled boutons is discontinuous during mild depolarization. (A) Ensemble average of boutons labeled as in Fig. 1 D with destaining stimulated by 12 mM K⁺. Data from 115 boutons from four coverslips. (B) Fluorescence traces from individual boutons reveal stepwise drops in fluorescence intensity. (C) A gallery of large (>14 fluorescence units) stepwise destaining events. (D) A gallery of small (<10 fluorescence units) stepwise destaining events.