Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Mar 14;168(6):887–897. doi: 10.1083/jcb.200408128

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 6. — The subcellular distribution of CaM-YFP was not changed by 10- or 1-Hz electrical stimulation. (A) A typical living fiber expressing CaM-YFP is shown for 30 min (−30 and 0) before stimulation and 60 and 120 min after repetitive stimulation with 10-Hz trains. In resting fibers, CaM-YFP was present in the cytoplasm as well as the nucleus. (B) The fluorescence signals from both the nucleus and the cytoplasm were quantitated. Data are presented as the ratio of the average fluorescence intensity per pixel from the nucleus relative to the cytoplasm. Neither 10-Hz train stimulation (left) for 2 h nor 1-Hz continuous stimulation (right) resulted in any subcellular redistribution of CaM-YFP. (C) FRAP of CaM-YFP was performed in the nuclear area of a muscle fiber. Shown is a nucleus before photobleaching, immediately after photobleaching, and after 30 and 60 min of recovery. The entire nuclear region was selectively bleached. Bars, 10 μm.