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. 2005 Mar 14;168(6):887–897. doi: 10.1083/jcb.200408128

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Copyright © 2005, The Rockefeller University Press

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Figure 9. — Effects of protein phosphatase inhibitors on the subcellular distribution of HDAC4-GFP. (A) The PP1 and PP2A inhibitor calyculin A dramatically decreased nuclear HDAC4-GFP in a 60-min period. HDAC4 nuclear and cytoplasmic fluorescence were constant during 30 min before calyculin A application. Calyculin A (final concentration 1 μM) caused a decline of nuclear fluorescence, but the cytoplasmic fluorescence remained constant. (B) 60-min exposure to the PP2B inhibitor CsA (1 μM) had no effect on nuclear fluorescence, whereas calyculin A subsequently decreased nuclear fluorescence in the same group of fibers.