Table I. Quantification of open and closed chromatin structures.
| Protein Expressed |
Decondensed (open) chromatin |
Condensed (closed) chromatin |
Indeterminate | Number of cells examined |
|---|---|---|---|---|
| % | % | % | ||
| CgCdc45 | 75 | 8 | 17 | 206 |
| CgCdc6 | 7 | 41 | 36a, 16 | 134 |
| Lac-DBD | 7 | 69 | 24 | 169 |
| Lac-DBD(2) | 12 | 73 | 15 | 138 |
| VP16 | 45 | 37 | 18 | 190 |
| BRCA1(6c-w) | 34 | 44 | 22 | 210 |
Proteins were expressed from pRcLac, except VP16 and Lac-DBD(2) (DNA-binding domain), which were from pNYE4.
a
The number of LacI-Cdc6–expressing cells displaying a homogenous nuclear stain with no apparent closed or open structures.