Table II. Frequency of colocalizing proteins/modifications with targeted fusion proteins.
| LacI fusion targeted | Colocalizing protein/modif. |
Percent observed | |
|---|---|---|---|
| Cdc45 | + | H1P | 65% |
| Cdc45 (closed) | + | H1P | 0% |
| Cdc45 | + | Cdk2 | 25% |
| Cdc45 (closed) | + | Cdk2 | 0% |
| Cdc45 | + | AcH3 | 0% |
| Cdc45 | + | AcH4 | 0% |
| Cdc45 | + | MeK4 | 0% |
| VP16 | + | H1P | 0% |
| LacI (closed) | + | H1P | 0% |
| VP16 | + | AcH3 | 75% |
| VP16 | + | AcH4 | 75% |
| VP16 | + | MeK4 | 50% |
| Cdk2-wt | + | H1P | >80%a |
| Cdk2-DN (open or closed) | + | H1P | 0%a |
Unless noted, colocalizations were observed with open chromatin structures; 50–60 cells were examined in each case.
a
The number of cells with very strong H1P colocalization.