Table I. Syd association with membranes is increased by sciatic nerve ligation.

Nerve axoplasm	Labeling	Membrane-bound gold particles/total gold
Proximal	syd labeling	92.9% (522 out of 562)
	control	35.3% (6 out of 17)
Distal	syd labeling	85.3% (133 out of 156)
	control	33.3% (5 out of 15)
Unligated	syd labeling	51.4% (19 out of 37)
	control	27.3% (3 out of 11)

Sciatic nerve ligation was performed as in Fig. 3. Nerves were dissected and processed for immunoelectron microscopy. Staining and counting was performed as described in Fig. 3 D. The percentage of the membrane-bound label, i.e., gold particles within a distance of 15 nm or less from the closest membrane profile, was determined.