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. 2005 Feb 28;168(5):747–759. doi: 10.1083/jcb.200412003

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Copyright © 2005, The Rockefeller University Press

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Figure 1. — SiRNA-induced COG3 KD is destabilizing Lobe A COG complex subunits. (A) Expression of COG subunits after COG3 KD. WB of cell lysates from control and COG3 KD cells. Average levels of the COG subunits (±SD, n = 4) after 72 h of COG3 KD were determined by quantitative WB, and normalized to mock-transfected cells. (B) Membrane localization of COG complex subunits. WB of membrane (P100) and cytosol (S100) fractions. (C) Cog6p localization. Control and COG3 KD cells that stably express GalT-GFP were fixed and analyzed by three-color IF microscopy after immunostaining with anti-Cog6p. DNA was stained with DAPI. Arrows indicate Golgi or Golgi fragments. Bars, 10 μm.