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. 2005 Feb 28;168(5):747–759. doi: 10.1083/jcb.200412003

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Copyright © 2005, The Rockefeller University Press

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Figure 7. — Accumulation of CCD vesicles precedes COG3 KD-induced Golgi fragmentation. Control or COG3 KD cells that stably express GalT-GFP were fixed 48 h after siRNA transfection and stained with anti-GS15 and secondary antibodies conjugated with Alexa 594. Images were acquired with 63× objective and deconvolved. Note that the Golgi (arrows) has not yet been fragmented in a subpopulation of the COG3 KD cells, whereas the majority of GS15 was associated with multiple CCD vesicles. Bar, 10 μm.