Table IV. The constitutive active 1*6 STAT5A protein induces adhesion-independent cell cycle progression.

	G0/G1	S	G2/M
	%	%	%
(HEK293 IL-3R + Neo   vector) S	63.3 ± 8	29.2 ± 4	7.5 ± 1.5
(HEK293 IL-3R +   1*6 STAT5A) S	53.2 ± 7	42 ± 5	4.8 ± 2
(HEK293 IL-3R +   1*6 STAT5A) S + IL-3	52.3 ± 5	44.9 ± 4	2.9 ± 3
(HEK293 IL-3R +   1*6 STAT5A) FN	57.5 ± 6	40.2 ± 3	2.3 ± 2
(HEK293 IL-3R +   1*6 STAT5A) FN + IL-3	58 ± 5	39.7 ± 4	2.3 ± 2

HEK293 IL-3R cells, transfected with the empty vector (Neo vector) or with the constitutive activated STAT5A construct (1*6 STAT5A), were serum starved for 24 h and kept in suspension or let to adhere to FN-coated plates in the presence or absence of 20 ng/ml IL-3. Cell cycle progression was assayed on ethanol-fixed cells. DNA was stained with propidium iodide. The fluorescence related to DNA content was evaluated as described in Table II. The numbers are the mean ± SD of three different experiments done on separate days, each performed in triplicate.