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. 2005 Mar 28;168(7):1039–1051. doi: 10.1083/jcb.200409138

Figure 3.

Figure 3.

Identification of critical amino acids in residues 101–116 of Arf-1 for Golgi targeting of the Arf-6-1-6 chimera. (A) Sequence comparison of residues 101–116 of Arf-1 and corresponding residues of Arf-6 and mutants of Arf-6-1-6 are shown. Bold characters indicate residues unique to Arf-6. (B) COS-7 cells overexpressing GalT-GFP and the Arf constructs indicated were fixed and immunolabeled with antibodies against Arf-6 followed by Alexa 594 anti–rabbit antibodies. 100 cells were counted and the fraction of transfected cells that show clear colocalization of overexpressed Arf proteins with GalT-GFP was noted. The result shown is the mean ± SD for three experiments. (C) Sequence comparison of residues 101–116 of Arf-1 and corresponding residues of related proteins are shown. Bold characters indicate residues identical to 110M and 113E of Arf-1.