Figure 4.

Amino acid residues 101–116 of Arf-1 are targeted to the Golgi. (A) Schematic representation of GFP-Arf-1(101–116). (B–D) HeLa cells expressing GFP-Arf-1(101–116) were fixed and immunolabeled with antibodies against β-COP (B and D) or GM130 (C) followed by Alexa 594 anti–rabbit and anti–mouse antibodies, respectively. Cells expressed GFP-Arf-1(101–116) at low to moderate level (B) or high level (C and D). The transfected cells in C and D are outlined. Images I and II in C show magnified views of boxed areas. Bars, 10 μm.