Figure 7.

Anterograde movement of membrin and Arf-1 into the Golgi in BFA-recovering cells. (A) NRK cells overexpressing myc-membrin and Arf-1-GFP or Arf-1-6-1-GFP were incubated in the presence of 5 μg/ml BFA for 30 min, washed free of BFA, and incubated in the absence of BFA for the indicated times of wash out (WO) and fixed. Cells were immunolabeled with antibodies against myc followed by Alexa 594 anti–mouse antibodies. (B) NRK cells overexpressing myc-membrin were treated as in A and fixed. Cells were immunolabeled with antibodies against myc and β-COP followed by Alexa 488 anti–mouse and Alexa 594 anti–rabbit antibodies. Bars, 10 μm.