Figure 1.

GMAP-210 targeting to the Golgi. A schematic depiction of human GMAP-210 showing the predicted segments of coiled-coil (green), the GRAB domain (blue) and GRAB-associated region (GA1; red), and the proline-rich hydrophobic sequence (yellow). Nomenclature is that which has been used previously (Gillingham et al., 2004). The data of Gillingham and Munro (2003) and Chen et al. (1999) are summarized in the top panel. They identified a minimal Golgi-targeting region between amino acids 1757 and 1838, the GRAB domain. Deletion of 1754–1866 aa, removing the GRAB domain, or mutation of the conserved aspartate 1780 and leucine 1783 to alanine abolishes Golgi targeting of GMAP-210 (Chen et al., 1999; Gillingham et al., 2004). Neither group found a role for the NH₂ terminus in Golgi targeting. The data of Rios et al. (2004) and Infante et al. (1999) are summarized in the bottom panel. They described 1–375 aa as a Golgi-targeting region and 1797–1979 aa as a MT- and γ-tubulin complex–binding domain.