Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Apr 25;169(2):321–329. doi: 10.1083/jcb.200412145

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — Localization and production of F-actin in response to cAMP are impaired in chemotactically competent Ddnhe1 ⁻ cells. (A) In Ax2 cells (top), F-actin was localized predominantly at the leading edge of the cell, and F-actin-rich protrusions were limited to the direction of the chemoattractant source (asterisks). In Ddnhe1 ⁻ cells (bottom), F-actin was distributed along the cell cortex, and although membrane protrusions (arrowheads) also contained F-actin, protrusions were not limited to the direction of the chemoattractant source but were seen around the cell. (B) Time course of F-actin production in response to a uniform concentration of 2 μM cAMP indicated a characteristic biphasic response in Ax2 cells (blue line), including a rapid (<10 s) first phase of greater magnitude and a slower and smaller second phase. In Ddnhe1 ⁻ cells (black line), the increase in F-actin in the first phase was reduced by ∼60% and the second phase was transient with reduced F-actin formation, compared with Ax2 cells. Ddnhe1 ⁻ /DdNHE1 cells (red line) had a biphasic increase in F-actin, although the magnitude of first and second phases was slightly greater compared with Ax2 cells. Data are expressed as the mean ± SEM of at least three independent cell preparations.