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. 2005 Apr 25;169(2):355–366. doi: 10.1083/jcb.200411012

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Copyright © 2005, The Rockefeller University Press

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Figure 8. — Overexpression of LL5β perturbs AChR clustering. Myoblasts cultured on laminin (Fig. 6) were transfected with the indicated constructs, fused to form myotubes, and then stained with rBTX. (A) Few large AChR clusters are present in myotubes that overexpress GFP-LL5β; instead, receptors are present diffusely and in small patches (arrows). (B and C) Forced expression of GFP (B) or a GFP-LL5β fusion protein lacking the PH domain (GFP-LL5β-ΔPH; C) has no effect on the frequency of aggregation. Bar, 100 μm. (D) Percent of transfected (strongly fluorescent) or untransfected myotubes with complex AChR aggregates. 80 myotubes were analyzed per condition: 40 in each of two separate experiments. Difference between GFP-LL5β and each other condition is significant at P < 0.001. Error bars show mean ± SEM.

Figure 8. — Overexpression of LL5β perturbs AChR clustering. Myoblasts cultured on laminin (Fig. 6) were transfected with the indicated constructs, fused to form myotubes, and then stained with rBTX. (A) Few large AChR clusters are present in myotubes that overexpress GFP-LL5β; instead, receptors are present diffusely and in small patches (arrows). (B and C) Forced expression of GFP (B) or a GFP-LL5β fusion protein lacking the PH domain (GFP-LL5β-ΔPH; C) has no effect on the frequency of aggregation. Bar, 100 μm. (D) Percent of transfected (strongly fluorescent) or untransfected myotubes with complex AChR aggregates. 80 myotubes were analyzed per condition: 40 in each of two separate experiments. Difference between GFP-LL5β and each other condition is significant at P < 0.001. Error bars show mean ± SEM.