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. 2005 Apr 25;169(2):297–308. doi: 10.1083/jcb.200410119

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Copyright © 2005, The Rockefeller University Press

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Figure 4. — Strong nuclear oscillations in mto2Δ cells. (A) Mto2Δ cells expressing nup107-GFP and alp4-GFP (PT574). Red dots indicate the center of the nucleus. The SPB (white arrowhead) oscillates in response to polymerization of attached MTs. Imaging: five collapsed wide field image planes spaced 1 μm apart. 1-min time interval. Bar, 5 μm. (B) Nuclear displacements, projected along the long axis of the cell, in three nup107-GFP expressing cells. Shown are traces for a wild-type cell (green; PT53), an mto2Δ cell that showed large extensions of the nuclear membrane (solid red; PT510) and an mto2Δ cell without such extensions (hatched red; PT510). (C) SDs were calculated for many curves similar to B. Shown is the distribution of SDs for wild-type (green, n = 68 cells) and mto2Δ (red, n = 76) cells. The distribution is subdivided for mto2Δ cells that did (solid, n = 45) and did not show (hatched, n = 31) large membrane extensions.