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. 2005 Apr 25;169(2):297–308. doi: 10.1083/jcb.200410119

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Copyright © 2005, The Rockefeller University Press

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Figure 6. — Mto2p-dependent MT nucleation after MBC washout. Imaging: projected confocal planes showing the complete cell depth. (A) Wild-type cell expressing alp4-GFP and GFP-tubulin (PT546). After the addition of 25 μM MBC, at t₀, MTs depolymerize to four short stubs (marked in red); one of them is connected to the SPB visualized by alp4-GFP. Two stubs disappear during the prolonged 10-min MBC incubation. After MBC washout, at t₁, MT regrowth occurs from stubs (red arrowheads) and five new sites. (B) mto2Δ cell expressing alp4-GFP and GFP-tubulin (PT541). The bundle hardly shortens after MBC treatment, indicating a long stabilized region. Treadmilling MTs (red) first disappear but later reappear from MT breakage events (green arrowhead). Yellow arrowheads mark the SPB. Bar, 5 μm.