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. 2005 Apr 25;169(2):341–354. doi: 10.1083/jcb.200409127

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Copyright © 2005, The Rockefeller University Press

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Figure 3. — Targeting of NCAM to lipid rafts is reduced in PrP ^−/− mice. (A) Brain homogenates or lipid rafts from brain homogenates and growth cones of PrP^+/+ and PrP^−/− mice (0–4 d old) were immunoblotted (WB) with antibodies against NCAM. The band at and above 180 kD represents polysialylated NCAM140 and NCAM180 (PSA-NCAM; Niethammer et al., 2002). In lipid rafts, a band representing nonpolysialylated NCAM120 is also observed. Diagrams show quantitation of PSA-NCAM and NCAM120. Note that the amount of PSA-NCAM is increased in brain homogenates and decreased in rafts isolated from brain homogenates and growth cones of PrP^−/− mice. (B) Lipid rafts isolated from brain homogenates of PrP^+/+ and PrP^−/− mice (0–4 d old) were immunoblotted with L1 antibodies. Diagrams show quantitation of L1. Note that the amount of L1 is similar in rafts isolated from PrP^−/− and PrP^+/+ mice. Mean values ± SEM (n = 6) are shown. AU = arbitrary units. *, P < 0.05, paired t test (for A and B).