Figure 6.

Aβ induces forkhead phosphorylation. (A) In SH-SY5Y cells treated with 30 μM Aβ at various times after a 16-h serum starvation period, endogenous FKHRL1 phosphorylation was detected by Western blot analysis using antibodies that recognized phospho-FKHRL1 or FKHR. (B) In 7 DIV cultures treated with 25 μM Aβ for various lengths of time, endogenous FKHR and phosphorylated FKHR were detected by Western blot analysis using antibodies that recognized phospho-FKHR or FKHR, respectively. The blots are representative of three separate experiments. (C) Endogenous FKHR phosphorylation in transfected PC12 cells that ectopically expressed either p66Shc or p66ShcS36A after treatment with Aβ was assessed by Western blotting using antibodies that recognized either phospho-FKHR or FKHR. These experiments were performed at least three times, yielding the same results.