Figure 7.

The recruitment of myosin VI to tubular vesicular structures formed after overexpression of the constitutively active form of Rab8 is lost in optineurin-depleted cells. The constitutively active form of Rab8 (GFP-Rab8-Q67L) overexpressed in NRK cells localizes with myosin VI at the Golgi complex (a and b) and recruits myosin VI onto tubular and vesicular structures emerging from the Golgi complex (e and f). In optineurin-depleted NRK cells, which are overexpressing GFP-Rab8-Q67L myosin VI no longer colocalizes with Rab8-Q67L at the Golgi complex (c and d) and is not recruited to tubular and vesicular structures positive for Rab8-Q67L (g and h). White boxes highlight the areas of the cells enlarged in the pictures below (e′,f′, g′, and h′). Endogenous myosin VI was detected with either a pAb to the globular tail (a, c, g, and g′) or to the whole tail (e and e′). Bars, 10 μm.