Table III. Oligonucleotide primers used in this study.
| Primer | Mutation | Sequence (5′ → 3′) |
|---|---|---|
| N323 | R361K CPYΔ1 | gccggtcttttggtaaggagccaa |
| N324 | R364K CPYΔ1 | aagaacgtttacgataacgaatgg |
| N325 | K493R CPY* | gtaggatctgacttcaccagcgac |
| N326 | K496R CPY* | agacacttcacctatttgagagtc |
| N335 | K448R CPY* | acagatgaaatctctatcgcctgc |
| N336 | K458R CPY* | aactggttgggtaatcgggcgtgg |
| N337 | K467R CPY* | ttcgtcgtaccgccatggcaagac |
| N338 | K476R CPY* | gaatttgcaagccaacgagtacgt |
| N339 | R276K CPYΔ1 | gtccttgtgagacaaaatttcaga |
| N340 | R276K CPYΔ1 | aaaaacttcaacttaacctccgtc |
| N435 | K371R CPY* | cctgatatcgtaaacgtttctgcc |
| N436 | K371R CPY* | agagattgtgaaggtggcaatttg |
| N437 | K396R CPY* | gacgtagtcctggtttaagtagtc |
| N438 | K396R CPY* | agagaagctgtcggtgcggaggtt |
| N439 | K425R CPY* | catccaatcacccgcaaacaggaa |
| N440 | K425R CPY* | agaccttaccacaccgccgtaaca |
| N441 | N479Q CPY* | acgtactttttggcttgcaaattc |
| N442 | N479Q CPY* | cagtggactgcttctatcaccgac |
| N491 | N124Q CPY* | tgggtcaatgcccaggattttagg |
| N492 | N124Q CPY* | caggtcacacagtacacgggttac |
| N493 | N198Q CPY* | gctgttccaagagtaagggttccc |
| N494 | N198Q CPY* | caggccaccgtgatcttccttgac |
| N495 | N279Q CPY* | gaagtttctgtccttgtgagacaa |
| N496 | N279Q CPY* | cagttaacctccgtcttgatcgga |
| N527 | N107Q PrA* | agctttgtagcttgatgaagcttc |
| N528 | N107Q PrA* | cagggtactgaatttgccattcaa |
| N537 | N308Q PrA* | attgaagttgaaaattagat |
| N538 | N308Q PrA* | ggctaccagttcactattgg |