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. 2005 Apr 11;169(1):139–149. doi: 10.1083/jcb.200412162

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Copyright © 2005, The Rockefeller University Press

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Figure 9. — Effect of PIPKI overexpression on phagocytosis. RAW cells transfected with the plasmids indicated below were exposed to IgG-opsonized particles for 8–10 min, then analyzed by laser confocal microscopy either immediately (A and B) or after fixation and staining with Alexa 633-phalloidin (D) or rhodamine-phalloidin (E and F). (A and B) RAW cells transiently transfected with PIPKIβ-YFP and PH^PLCδ-CFP. (C and D) Cells transiently transfected with PIPKIβ-YFP. (E and F) Cells transiently transfected with PIPKIγ-GFP. (G) Quantification of the phagocytic efficiency of untransfected cells (control) or cells transiently transfected with PIPKIα-CFP, PIPKIβ-YFP, or PIPKIγ-GFP. Data are the means ± SEM of three experiments, each scoring at least 100 cells.