Figure 5.

Coll/FN mixed matrices. BAE cells were plated on increasing amounts of FN with or without precoating with 10 μg/ml Coll. (A) Cells were sheared for 30 min and nuclear p65 accumulation was assessed as previously described. The abscissa represents the amount of FN adsorbed to the coverslips under these conditions. Values reflect the increase in p65 nuclear translocation relative to cells under static conditions. n = 4. (B) Cells were treated as in A, and p65 phosphorylation on Ser536 was assessed by Western blotting. Bands were quantified by densitometry and normalized to total p65. Values represent the increase in p65 phosphorylation relative to cells under static conditions. n = 3. (C) Cells were exposed to shear stress for 5 min and p38 phosphorylation was assessed by Western blotting. Bands were quantified by densitometry and normalized to total p38, and data was expressed as the fold increase in p38 phosphorylation compared with cells under static conditions. n = 3.