Figure 6.

PKCη is required for protein transport from TGN to the cell surface. HeLa cells were transfected with GFP-tsO45 VSV-G and FLAG-PKCη-wt or FLAG-PKCη-kinase dead. Following the procedure described in Materials and methods, immunofluorescence microscopy was used to detect GFP-tsO45 VSV-G localization. VSV–G protein is retained in the Golgi apparatus in PKCη kinase dead–expressing cells after 120 min incubation at the permissive temperature C (e–h), compared with cells expressing the wild-type PKCη (a–d).