Table II. Embryonic lethality due to depletion of UNC-116 and associated proteins.
| Genotype | Embryonic lethality | Brood size |
|---|---|---|
| % | ||
| wild type | 3.3 ± 4 (9,999/95) | 174.4 |
| wild type + males | 2.6 ± 0.4 (4,509/28) | 241.6 |
| unc-116(RNAi) | 66.9 ± 19 (2,230/45) | 71.8 |
| unc-116(RNAi) + males | 65.3 ± 26 (1,904/16) | 161.6 |
| unc-116(rh24,sb79) | 27.8 ± 9 (1,099/23) | 57.5 |
| unc-116(rh24,sb79) + males | 24.0 ± 9 (800/24) | 40.4 |
| unc-116(rh24,sb79, RNAi) | 72.5 ± 6 (560/24) | 28.5 |
|
unc-116(rh24,sb79, RNAi)
+ males |
58.2 ± 11 (756/23) | 39.2 |
| klc-1(RNAi) | 16.4 (785/10) | 156.1 |
| klc-1(RNAi) + males | 11.7 (725/9) | 132.2 |
| klc-2(RNAi) | 1.7 (879/9) | 166.1 |
| klc-2(RNAi) + males | 4.3 (836/7) | 155.3 |
| klc-1(RNAi); klc-2(RNAi) | 65.8 (912/17) | 113.4 |
|
klc-1(RNAi); klc-2(RNAi)
+ males |
64.6 (1,584/10) | 221.6 |
| C10H11.10/kca-1(RNAi) | 89.2 ± 9 (1,719/54) | 73.4 |
| C10H11.10/kca-1(RNAi)
+ males |
69.0 ± 20 (2,660/30) | 121.7 |
Embryonic lethality is the percentage of eggs that did not hatch within 12 h. Numbers in parentheses indicate the number of embryos counted/number of worms tested by RNAi soaking. + males indicates that male worms were added to provide wild-type sperm. Brood size is the average number of eggs laid by a single worm in 48 h. Wild-type is the GFP-tubulin–expressing strain WH204.