Figure 1.
Generation of Atg7F/F mice. (A) Schematic representation of the targeting vector and the targeted allele of Atg7 gene. The coding exons numbered in accordance with the initiation site as exon 1 are depicted by black boxes. Green and red boxes indicate Atg7 cDNA fragment (aa 1786–2097) and Atg7 cDNA fragment (aa 1669–1698) with stop codon, respectively. The open triangles denote loxP sequence. A probe for Southern blot analysis is shown as a gray ellipse. Arrows indicate the positions of PCR primers. The asterisk denotes the essential cysteine residue on exon 14. EcoRV, EcoRV sites; neo, neomycin-resistant gene cassette; DT-A, diphtheria toxin gene. (B) Southern blot analysis of genomic DNAs extracted from mice tails. Wild-type and Flox alleles are detected as 17.5- and 7.5-kb bands, respectively. (C) Immunoblot of Atg7 in MEFs. The lysates of MEFs of indicated genotypes were immunoblotted with Atg7 and actin.