Figure 3.

Regulation of c-Myc/Mnt target genes during cell cycle entry in the absence of Mnt. RNA from wild-type and Mnt null primary MEFs was harvested at the indicated times after serum stimulation and quantitative real-time RT-PCR assays performed in triplicate for the indicated genes. The data are representative of two or more independent experiments. RNA levels of the ARBP P0 gene, which do not change during cell cycle entry (not depicted), were used to standardize samples. Fold expression values were calculated relative to the 0-h time point in wild-type cells for each gene as previously described (Livak and Schmittgen, 2001). SDs are shown.