Figure 3.

Suppression of Drosophi la CRAC current by Stim dsRNA treatment. (A) Current development evaluated at −110 mV in selected control cell (untreated). Cells were bathed in S2 external solution with 2 mM Ca²⁺ and dialyzed with BAPTA-buffered S2 internal solution to induce store depletion passively. Whole-cell recording was initiated at time 0. (B) Leak-subtracted current-voltage relation of maximal Drosophila CRAC current recorded in the same control cell. (C) Typical Stim dsRNA-treated cell; current at −110 mV. (D) Leak-subtracted I-V relation 200 s after establishing the whole cell configuration. (E) CRAC current density in dsRNA-treated and untreated S2 cells. Each point represents CRAC current density (pA/pF) in a single cell, plotted in consecutive order from left to right within six groups of cells: untreated (circles, n = 27 cells in three experiments); cells treated with dsRNA to suppress CG11059 (triangles, n = 21 cells in three experiments); CG1560 (diamonds, n = 45 cells in six experiments); trp-l (inverted triangles, n = 20 cells in two experiments); CG8743 (pentahedrons, n = 16 cells in two experiments); or Stim (squares, n = 77 cells in eight experiments). Groups 1, 2, and 5 include one cell each with current density >6 pA/pF. Horizontal lines indicate the mean value of current density in each group.