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. 2005 May 9;169(3):481–489. doi: 10.1083/jcb.200412069

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Figure 2. — Traffic of GLUT4 vesicles in adipose cells in the basal and insulin-stimulated states. Time-lapse TIRF images were projected onto a single plane to visualize vesicle movements. (A) Projection image of a cell in the basal state made from a 1-min-long recording (Video 1, available at http://www.jcb.org/cgi/content/full/jcb.200412069/DC1). (B) Sequential frames show three vesicles (arrows) taking the same pathway (white line). See Video 2. (C) Projection image of a cell taken 10 min after insulin application. Note that both the number of traces and the average running distance for GLUT4 vesicles are significantly reduced compared with the basal state. Fluorescence intensity is shown in pseudocolor. (D) Quantification of traffic for cells in the basal state and cells 5 and 10 min after insulin application. Data represent mean number of traces detected per 100 μm²/min from at least 20 cells in each state.