Figure 7.
Localization of KKSL-tagged caveolin-1 mutants. FRT cells expressing Ins-7L1-KKSL (A–C), 97/SASA-KKSL (D–F), Ins-7L(1+2)-KKSL (G–I), or Ins-7L(1+2)+Δ112-125-KKSL (J–L) were methanol fixed. Caveolin-1 proteins (A, D, G, and J) and ADRP in the same cells (B, E, H, and K) were detected by IF. (C, F, I, and L) Merged images. Schematic depictions of proteins are as in Fig. 3, except that only two triangles indicate the four substitutions in 97/SASA. *, KKSL tag.