Figure 3.

Kinetochore association of spindle checkpoint proteins in nocodazole-treated cells. (A) ChIP of Mad1p-GFP, Mad2p-GFP, and Mad3p-GFP at CENIV in cycling and nocodazole-treated cells. Graphs are as described for Fig. 1 (D–G). (B) Wild-type cell coexpressing the SPB protein Spc42p-CFP and the kinetochore protein Ndc80p-GFP after treatment with 25 μg/ml nocodazole for 1 h at 25°C. Panels show Ndc80p-GFP alone, Spc42p-CFP alone, and Spc42p-CFP (red) merged with Ndc80p-GFP (blue). (C–E) Wild-type cells coexpressing Ndc80p-CFP (blue) and Mad1p-GFP, Mad2p-GFP, or Bub1p-GFP (green) after nocodazole treatment. Panels show CFP channel alone, GFP channel alone, and CFP merged with GFP. Orange arrowheads indicate the locations of unattached kinetochores.