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. 2004 Feb 16;164(4):501–507. doi: 10.1083/jcb.200310014

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — Aging yeast cells show caspase activation and release substances that can stimulate survival of other old cells. (A) Chronologically aged wild-type and yca1 disruption strains were analyzed in vivo for caspase activity by FITC-VAD-fmk staining using flow cytometry for quantification. This experiment was performed three times independently with similar results. (B) Survival of chronologically aged wild-type yeast in the presence of secretions released by 1- or 8-d-old cultures (final concentration 2 mg/ml, addition on d 7 and 11) or without addition. Experiments were performed three times independently with different secretions and gave similar results.