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. 2004 Feb 16;164(4):493–499. doi: 10.1083/jcb.200309082

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Copyright © 2004, The Rockefeller University Press

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Figure 5. — MOMP and inhibition of mitochondrial fusion. (A) HeLa cells were cotransfected with mito-PAGFP (pseudocolored), Smac/DIABLO-CFP (red), and pCDNA-Bax, treated with 75 μM zVAD-fmk, and, after addition of 1 μM STS, followed by sequential photoactivation of selected regions (a1, a2, and a3) and acquisition of serial images. Note the close temporal correlation of MOMP (i) and mitochondrial fusion inhibition. Brightness of the Smac/DIABLO-CFP images in panels i–m has been increased to visualize the release of protein more clearly. Insets show magnified mito-PAGFP fluorescence within the photoactivated area of the cell. (B) Changes in the average fluorescence intensities of mito-PAGFP in different cells after STS addition and serial photoactivations as in A. (C) Increase in homogeneity of Smac/DIABLO-CFP, reflecting release from mitochondria (see Materials and methods) within cells shown in B were measured over time and plotted against the time. The same color and pattern lines in B and C were obtained from the same cells. The cell shown in A is depicted by the green lines in B and C.