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. 2004 Sep 27;166(7):1055–1067. doi: 10.1083/jcb.200404002

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Copyright © 2004, The Rockefeller University Press

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Figure 8. — Adaptive regrowth and stress resistance of wild-type strain DBY746 and of regrowth mutants. (A–C) Survival and adaptive regrowth of wild-type cells and four different haploid regrowth mutants (adr-1, adr-3, adr-4, and adr-5). Representative experiments are shown. (D) Survival and adaptive regrowth of diploid DBY746/DBY747 (+/+) and heterozygous adr1/+ mutant. A representative experiment performed in duplicate is shown. (E) Heat-shock resistance of wild-type (DBY746) and regrowth mutants adr-1 and adr-3. Serial dilutions of day 3 postdiauxic phase cultures were spotted onto YPD plates and incubated at 55°C for 90 min. (F) Oxidative stress resistance of wild-type, adr-1, and adr-3 mutants. Day 3 postdiauxic phase cells were diluted in K-phosphate buffer and treated with 250 μM of menadione for 1 h. After treatment, cells were serially diluted, spotted onto YPD plates, and incubated at 30°C for 3–4 d. Both heat-shock and oxidative stress resistance experiments were repeated four times with similar results.