Table I.
Concentration of the U4 snRNP in CBs is not changed upon expression of mutant SART3 (ΔCT10ΔRRM)
| Expressed construct | Ratio of fluorescent intensities (CB/nuc.) ± SD (n)a | ||
|---|---|---|---|
| LSm4 (U6)b
|
U4 snRNA
|
61K
|
|
| None | 1.82 ± 0.51 (124) | 1.55 ± 0.27 (88) | 1.71± 0.44 (70) |
| SART3–GFP | 1.58 ± 0.40 (130) (P = 0.0011)c |
1.64 ± 0.47 (65) (P = 0.30)c |
1.89 ± 0.53 (53) (P = 0.079)c |
| ΔCT10ΔRRM–GFP | 1.28 ± 0.27 (157) (P ≪ 10−10)c |
1.79 ± 0.42 (112) (P = 0.0007)c |
1.72± 0.49 (77) (P = 0.90)c |
a
Ratio of average fluorescent intensity/pixel in CB and the nucleoplasm (nuc.); n = number of CBs evaluated.
b
Data taken from Stanek et al. (2003).
c
P values determined from the t test comparing fluorescent intensity ratios from the experimental data sets versus control ratios (shown in “None” row).