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. 2004 Apr 26;165(2):175–180. doi: 10.1083/jcb.200312118

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Copyright © 2004, The Rockefeller University Press

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Figure 4. — PKA activation abolishes the inhibitory effect of Rab13Q67L on the recruitment of ZO-1 and claudin1. Cells expressing GFP, GFP-Rab13T22N, or GFP-Rab13Q67L were grown without Ca²⁺ for 16 h and subsequently incubated in normal medium for the time indicated in the absence or presence of Forskolin (Fsk). The localization of (A) ZO-1 and (B) claudin1 was analyzed by immunofluorescence. In cells expressing Rab13Q67L, the recruitment of ZO-1 and claudin1 to cell–cell junctions was delayed. Forskolin treatment restored ZO-1 and claudin1 recruitment in those cells. Bars, 10 μm.