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. 2004 Apr 26;165(2):233–242. doi: 10.1083/jcb.200311040

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Copyright © 2004, The Rockefeller University Press

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Figure 7. — The fascin-mediated elongation phase is independent of Arp2/3-nucleating activity. (A) CA inhibits Arp2/3-dependent nucleation, but does not affect fascin-mediated elongation. (B) AMPPNP inhibits Arp2/3-dependent nucleation, but does not affect fascin-mediated elongation. Each point in A and B represents the average of at least 66 bacteria from two experiments ± SD. (C) Arp2/3 localizes to the portion of the comet tail assembled during the nucleation phase, but is not found on the tail assembled during elongation or at the bacterial surface. Bar, 10 μm.