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. 2004 Apr 26;165(2):233–242. doi: 10.1083/jcb.200311040

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Copyright © 2004, The Rockefeller University Press

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Figure 9. — Rates of L. monocytogenes motility as a function of G-actin concentration under nucleation and elongation conditions. Bacteria were preincubated in either VASP or brain cytosol (step 1). To initiate motility, the preincubation solution was replaced with Arp2/3, actin, and capping protein (step 2). For motility in the absence of Arp2/3, the nucleating solution in step 2 was replaced with a solution containing actin, fascin, and CA (step 3). Motility rates in the Arp2/3-dependent reaction were analyzed in step 2. Rates in the fascin-dependent elongation reaction were analyzed in step 3. Each point is the average of at least 30 bacteria from three separate experiments ± SD.