Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2004 Apr 26;165(2):255–262. doi: 10.1083/jcb.200312033

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2004, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — Exocytic events triggered by photolysis of a caged-Ca² ⁺ compound. (A) SRB fluorescence image of an islet that had been preloaded with NP-EGTA and fura-2. (B) Fura-2 (Ca²⁺) images obtained 1.2 s before and after UV irradiation of the islet shown in A. (C) Representative time courses of normalized fura-2 fluorescence (black) and of [Ca²⁺]_i estimated with fura-2FF (blue). Fura-2 fluorescence (F) was normalized by the resting fluorescence (F₀), and the trace shown is for the islet in B. (D) Exocytic event (arrow) triggered by UV photolysis of NP-EGTA. Ω Profiles often persisted for tens of seconds. (E) Two examples of time courses of sequential exocytosis (blue and red) and an example of that of solitary exocytosis (black) triggered by photolysis of NP-EGTA. The traces were aligned at the onset of exocytosis. (F) Time courses of the decay of SRB fluorescence associated with exocytic events induced by 20 mM glucose (black and red) or by photolysis of NP-EGTA (green). The traces are averages of >10 exocytic events, which were aligned at the peak of SRB fluorescence and normalized by the peak intensity. The data correspond to solitary events (black and green) or to the primary event of sequential exocytosis (red).