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. 2004 Mar 29;164(7):1045–1054. doi: 10.1083/jcb.200308158

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Copyright © 2004, The Rockefeller University Press

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Figure 1. — Characterization of the Samba mutants, Mhc^S1 and Mhc^S2. (A) Temperature-sensitivity curves of several mutant strains that affect muscle function. TS behavioral defects are found only in hypercontraction mutants. (B) Thoraces of CantonS (left), eag ¹, Sh¹³³/Y (center), and Samba ¹/+ (right) adult male flies. (C) Schematic of a normal fly leg (left), a leg dissected from CantonS (center), and a leg showing femur hypercontraction from a Swg ^X118/+;Brkd ^J29/+ double heterozygote (right). Arrows indicate femoral segment. (D) Schematic depicting the genetic interactions of the Samba locus. (E–G) Polarized light micrographs showing hypercontraction of the IFM are shown in sagittal views of the thorax from adult flies of (E) CantonS, (F) Samba ¹/+, and (G) Samba ¹/+;Tm2 ^D53. Partial suppression is observed in Samba ¹/+;Tm2 ^D53, as IFM are found less degraded despite the presence of thoracic indentations (arrows).