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. 2004 Mar 29;164(7):985–996. doi: 10.1083/jcb.200301106

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Copyright © 2004, The Rockefeller University Press

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Figure 5. — Correlation of transcriptional activity with SC1's ability to block BrdU incorporation. NIH3T3 (A and B) and HEK293 (C and D) cells transiently transfected with Gal4SC1 fusion protein constructs. (A) SC1-expressing cells (green) are visualized by FITC-coupled anti-Flag antibodies. Bottom: the same field showing BrdU-labeled cells. (B) Histogram represents the percentage of cells transfected with Gal4, Gal4SC1FL, Gal4SC1Δ754–798, and Gal4SC1Δ583–798, which incorporated BrdU (mean and SD of at least three different experiments are shown). C and D are the same as A and B for HEK293 cells. Arrows indicate the cells transfected with SC1 constructs and the corresponding BrdU incorporation into their nuclei. Bars, 20 μm.