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. 2004 Mar 29;164(7):997–1007. doi: 10.1083/jcb.200312079

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — Detection and characterization of Sec61 complex oligomerization in situ. (A–C) Direct comparisons of FRET efficiencies to Sec61β_Cy5 from three different donor antibodies: Sec61β_Cy3 (at a ratio of 1:8; A), Sec61α_Cy3 (B), and 2°_Cy3 bound to unlabeled anti-Sec61α (C). (D) Quantitation (mean ± SD; n = 10) of the comparisons from A–C are shown.