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. 2004 Mar 29;164(7):1077–1087. doi: 10.1083/jcb.200401115

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Copyright © 2004, The Rockefeller University Press

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Figure 4. — Molecularly specialized nuclei cluster at AChR aggregates in aneural myotubes cultured on laminin. (A) DAPI-stained myonuclei (blue) cluster in apposition to branched aggregates (red); fewer nuclei are present elsewhere in the myotube (arrowheads). Dashed lines indicate myotube borders. (B) Nuclear density was quantified beneath an elliptical region defined by the extent of the AChR aggregate and in regions lacking AChR aggregates. The means of these distributions (± SEM) shows that average nuclear density is nearly threefold higher in association with aggregates (n = 230) than in AChR poor regions (n = 40). (C) Syne-1 (green) is more abundant in aggregate-associated nuclei (arrows) than in nuclei displaced from AChR aggregates (arrowheads). AChRs are red and nuclei blue, as in A. Dashed lines indicate the myotube borders. Bars: (A and C′′) 20 μm.