Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2004 Mar 29;164(7):1021–1032. doi: 10.1083/jcb.200309013

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2004, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — Mitochondrial targeting of Bax requires the 23 amino acids of the TMB region. (A) Anti-GFP Western blots of subcellular fractions from HEK293 cells expressing the indicated EGFP fusion constructs. To test the mitochondrial association of EGFP-23aa-C(Bax), isolated mitochondria were treated with sodium carbonate (pH 12) to remove loosely attached proteins (Att), and the pellet was extracted with detergent (right, Ins). (B) EGFP (green) and anti–mito marker (red) fluorescence analysis of HeLa cells transiently transfected with the indicated EGFP fusion constructs.