Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2004 Mar 29;164(7):1009–1020. doi: 10.1083/jcb.200310061

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2004, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — The YSK1-related kinase MST4 is Golgi apparatus localized. (A) Full-length YSK1, MST3, and MST4 were tested against empty vector (control), full-length GM130, GM130^1-271, and golgin45 for the ability to grow on selective medium (QDO), compared with nonselective medium (−LW) in the yeast two-hybrid system. Lighter colony color on QDO is an indicator of a strong signal. (B) His-tagged MST4 was incubated with GST-tagged fragments of rat GM130, golgin45, or GST and recovered on glutathione-agarose. Recovered complexes were analyzed by Western blotting. (C and D) HeLa cells transfected with myc-tagged MST3 and MST4 constructs for 18 h were costained with the 9E10 mAb to the myc epitope (green) and a sheep antibody to GM130 (red). Bar, 10 μm.